![]() ![]() Our findings suggest that homoeopathic dose (specially mother tincture) of Symphytum officinale has the potential to enhance osteogenesis.īone healing Gene expression Homoeopathy Mesenchymal stem cells Osteoblasts Osteogenesis Symphytum officinale Traditional medicine Asia & Oceania.Ĭopyright © 2019 Elsevier B.V. No known interactions with other medications, herbs, or supplements. Benefits and Features: A single active ingredient for highly targeted relief. The pellets melt in your mouth and can be taken on the go, no food or water needed. ![]() Interestingly, gene expression analysis for Runx-2 (n = 10), Osteopontin (n = 10), Osteocalcin (n = 10) and alkaline phosphatase activity (n = 8) also showed increased osteogenesis with the addition of Symphytum officinale to BDM, specially mother tincture. Symphytum Officinale 30C is a homeopathic dilution of Symphytum Officinale that relieves symptoms of bone trauma. The efficiency of osteogenic differentiation (2 weeks) for different doses of Symphytum officinale was determined by flow cytometry (n = 10) for osteoblast marker, Osteocalcin, and most doses of Symphytum officinale enhanced osteogenesis. MTT assay showed that the selected doses of SO did not induce any cytotoxicity in MSCs (24 hours). Gene expression analyses for osteoblast markers (Runx-2, Osteopontin and Osteocalcin) were evaluated in differentiated osteoblasts using qPCR.įlow cytometry (CD90, CD105) detected MSCs isolated from bone marrow (93-98%). Different homoeopathic doses of SO (MT, 3C, 6C, 12C and 30C) were added to the basic differentiation medium (BDM) and efficiency of MSCs differentiating into osteoblasts were measured by evaluating expression of Osteocalcin using flow cytometry, and alkaline phosphatase activity using ELISA. Osteogenic differentiation was induced in MSCs with β-glycerophosphate, ascorbic acid and dexamethasone over 2 weeks. Cytotoxicity of SO was evaluated by MTT assay. Comfrey, otherwise known as Knit-Bone, is a miracle plant and as its nickname suggests, is an herb esteemed for its ability to repair tissues & mend broken. ![]() MSCs were isolated, expanded and characterized by flow cytometry (CD90, CD105). This study aimed to determine if homoeopathic doses of SO could augment osteogenesis in MSCs as they differentiate into osteoblasts in vitro.īone marrow samples were obtained from patients who underwent bone grafting procedures (n = 15). Symphytum officinale (SO) is a medicinal plant and in homoeopathic literature, believed to accelerate bone healing. Mesenchymal stem cells (MSCs) are multipotent stem cells possessing regenerative potential. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |